PHYSICO CHEMICAL AND FUNCTIONAL PROPERTIES OF CHICKPEA PROTEIN ISOLATE
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Abstract
The main purpose of this research work was to isolate the most refined form of protein from chickpea for food processing. In this research work, chickpea (Cicer arietinum. L) was collected from Monywa Township, Sagaing Region and nutritional characteristics such as moisture content, ash content, fat content, carbohydrate content, protein content and fiber content were determined. The fat of raw bean flour was removed by bulk soaking in ethanol and also by soxhlet extraction using ethanol as solvent before isolating the protein. In addition, the fiber and starch from defatted chickpea flour was removed by alkaline extraction and acid precipitation method to isolate the protein (isoelectric precipitation). Protein solubility, water and oil absorption capacity, emulsifying capacity and stability, foaming capacity and stability of chickpea protein isolate have been determined. The solubility curve corresponding to the chickpea protein isolate indicated the minimum solubility at pH 4 (protein solubility of 24 %) and maximum solubility at pH 12 (protein solubility of 89 %) respectively. The chickpea protein isolate had water absorption capacity of 1.65±0.12 mL H2O/g protein and oil absorption capacity of 1.72±0.34 mL oil/g. protein. It was found that emulsion stability of isolated chickpea protein was 40.12 ±0.33 %with foaming capacity was 63.64±0.22 %. Isolated chickpea protein improved texture appearance and taste than the lentil flour and thus it can better be used as nutrition and functional ingredients in many food products.
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References
Abbas, S., M.S.B, M.T.S, M.K. S, A.N.A & R.B, “Nutritional and Functional Properties of Protein Isolates Extracted from Defatted Peanut Flour”International Food Research Journal, 22 (4), 153-1537, 2015.
AOAC, “Official Methods of Analysis of AOAC International”, 17th ed, Washington, DC: 5-15, 2000.
Aurelia, I., Iuliana,A., Aura, D., Gabriela, G., Cristina B. and Andrei,N., “Chemical and Functional Characterization of Chickpea Protein Derivates”, Journal of Food Technology, 5. 12-14. 2009.
Akaerue BI, Onwuka GI,“Evaluation of The Yield, Protein Content and Functional Properties of Mung Bean[ Vigna Radiate (L.) Wilczek] Protein Isolates as Affected by Processing”, Pakistan Journal of Nutrition, 9 (8).728-735, August, 2010.
Emami, “Hydrocyclone Fractionation of Chickpea Flour and Measurement of Physical and Functional Properties of Flour and Starch and Protein Fractions”, University of Saskatchewan, Saskatoon, Canada, 2007:1-347.
Eltayeb, A.R.S.M, Ali. O.A, A.A. Abou-Arab and F.M. Abu-Salem, “Chemical Composition and Functional Properties of Flour and Protein Isolate Extracted from Bambara Groundnut (Vigna subterranean)”, African Journal of Food Science, 5 (2), 82-90, February, 2011.
FAOSTAT: http// faostat.fao.org/site/567/default.aspx; last accessed 7 June 2007.
Garba, U, Kaur G, “Protein isolates: Production, functional Properties and applications”, International Research Journal of Chemistry (IRJC), 6 (3). 35-45.January, 2014.
George Amponsah, Zjen Ma & Joyce Irene Boye. “Crop- legume”, Food Processing: Principles and Applications, 305-337, 2014.
Ghribi AM, Maklouf I, Blecker C, Attia. H & Besbes .S, “Nutritional and Compositional Study of Desi and Kabuli Chickpea (Cicer Arietium L.) Flours from Tunisan Cultiviars, Advances food technology Nutritional Sciences, 1 (2):38-47, 2015.
Hui lui li, “Chickpea Proteins For Food Applications”,Ph.D thesis, University of Beijing , China, 1-3339, 1996.
Jayasena V.,Chih H.J, Nasar-Abbas S.M, “Efficient Isolation of Lupin Protein”, Food Australia,63 (7). 306-309, 2011.
Josefina Porras-Saavedra., Norma Guemez-Vera, Jose Luis Montanez-Soto, Maria Carmen Fernandez-Martinez and Jorge Yanez- Fernandez “Comparative study of functional properties of protein isolates obtained from three Lupinus species”, Advanced in Bioresearch, 4 (4). 106-116. December, 2013.
Ladjal Ettoumi,Y.. & Chibane .M. “Some Physico-Chemical and Functional Properties of Pea, Chickpea and Lentil Whole Flours”, International Food Research Journal, 22 (3):987-996, 2015.
Lawhon, J. T., C. M. Gater and K. F .Mattil, “Whippable Extract From Glandless Cottonseed Flour”, Journal of Food Science, 37, 317-321, 1972.
Lopez.O.Paredes, et al, “Chickpea Protein Isolates: Physicochemical, Functional and Nutritional Characterization”, Journal of Food Science: 726-729.56 (3), July, 2016.
Lin, M. J .Y. Humbert, E. S and Sosulki, F. W., “Certain Functional Properties of Sunflower Meal Products”. Journal of Food Science.39.368, 1974.
Mubarak AE., “Nutritional Composition and Antinutritional Factors of Mung Bean Seeds (Phaseolus Aureus) as Affected by Some Home Traditional Processes”, Journal of Food chemistry,89 :489-495 ,January, 2004.
Quayyum,M,M,N.,Butt,M.S.,Anjum, F.M.& Hawaz, H ,“Composition Analysis of Some Selected Legumes For Protein Isolate Recovery”, Journal of Animal and Plant Sciences, 22(4).1156-1162, 2012.
Recommended Daily Dietary Allowances, “Food and nutrition board”, National Academy of Sciences-National Research Council, USA, 1980.
Sathe, S .K., Deshpande, S .S. and Salunkhe, D. K., “Functional Properties of Winged Bean( Psophocarpus Tetragonolobus L.DC) Proteins”, Journal of Food Science, 47, 503-509, 1982
Seyam, A.A.,Banank,O.J & Breen,M.D, “Protein Isolates From Navy and Pinto Beans: their Uses in Macaroni Products”, Journal of Agricultural Food and Chemistry.31.499-502, 1983.
Shabnum Shaheen, Nidaa Harun,Farah Khan,Rana Abrar Hussian,Sehrish Ramzan,Sumaira Rani, Zaryab Khalid, Mushtaq Ahmad and Muhammad Zafar . “Comparative nutritional analysis between Vigna radiata and Vigna mungo of Pakistan”, African Journal of Biotechnology, 11 (25). 6694-6702. March, 2012.
Siddiq M, Ravi R, Harte J.B,Dolan K.D, “Physical and functional characteristics of selected dry bean (Phaseolus vulgaris L.)”, Food Science and Technology. 232-237, 2010.
Suliman, M. A., et al, “Solubility as influenced by pH and NaCl concentration and functional properties of lentil proteins isolate”. Pakistan Journal of Nutrition.5 (6).589-593, 2006.
Tharanathan, R.N. & Mahadevamma, S., “Grain Legumes A Boon to Human Nutrition”. Trends in Food Science and Technology, 14: 507-518, 2003.
Than Aung May, “Development and Adoption of Improved Chickpea Varieties in Myanmar”, 5(1): 1-3, December, 2007.
Withan Gamage Thushan Sanjeewa, “Physico-Chemical Properties of Chickpea Flour, Starch and Protein Fractions and their Utilization in Low-Fat Pork Bologn”, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, 1-223, August, 2008.
Volkert, M. A & B. P. Kelin, “Protein Dispersibility and Emulsion Characteristics of Flour Soy Products”. Journal of Food Science, 44, 43-96, 1979.