Research Protocol for Experimental Study of Acute and Sub chronic Dermal Toxicity of Mahabhallatak Tail in Wistar Albino Rats
DOI:
https://doi.org/10.69980/6t6kxw58Keywords:
Bhallataka, Semecarpus anacardium, Mahabhallataka Taila, Dermal toxicity, Ayurveda, Agadtantra, Wistar ratsAbstract
Ayurvedic medicinal preparations that contain Vishadravyas (toxic substances) like Bhallataka (Semecarpus anacardium Linn.) are popular in the treatment of chronic skin diseases. Although their therapeutic value is important, they may have potential toxicological risks during long-term use and thus require scientific evaluation based on modern parameters. One of the classic formulations, as shown in Kushtaroga, is Mahabhallataka Taila, which includes Bhallataka as one of its key ingredients, which has strong pharmacological and irritant effects. The objective of the current research is to determine acute and subchronic dermal toxicity of Mahabhallataka Taila in Wistar albino rats with an aim of determining its safety profile. The acute dermal toxicity was measured based on OECD guidelines with 14 days observation period and subchronic toxicity was measured based on repeated dermal application, 90 days observation and 28 days recovery. Such parameters as clinical symptoms, body weight, food consumption, hematological and biochemical indicators, and histopathology were evaluated. It is likely that the study will offer scientific data regarding the dermal safety, and assist in establishing the potential local or systemic toxicity of long-term use, which will support safe therapeutic use and the global acceptance and standardization of Ayurvedic forms.
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