Antimicrobial Activity of Biosynthesized Silver Nanoparticles Against Bacterial and Fungal Pathogens: A Comparative Experimental Analysis
DOI:
https://doi.org/10.69980/fkt2pj21Keywords:
Silver nanoparticles, Biosynthesis, Antibacterial activity, Antifungal activity, NanobiotechnologyAbstract
The biologically synthesized silver nanoparticles (AgNPs) offer a natural alternative for microbial pathogen management and offer alternative to traditional chemical synthesis. The results of this study showed that two AgNPs (S1_9 and S4_7) formulations prepared with Pseudomonas sp. metabolites exhibited comparable antimicrobial activities. Under various synthesis conditions, Z9.3 with various conditions. Six human-associated bacterial pathogens, three phytopathogenic bacteria and four fungal pathogens were analyzed in open experimental data at multiple levels of AgNP. Bacterial activity was measured as inhibition-zone and fungal responses as percentage growth inhibition compared to untreated controls. The effects of formulation, concentration, pathogen and formulation by concentration were evaluated by descriptive statistics and factorial analysis of variance. Both preparations showed an increase in antibacterial activity with increasing concentration and the mean inhibition zones provided by S1 were greater than S4. The species of bacteria and formulation and concentration were found to have significant effects on the antibacterial response and the formulation-by-bacterial-species interaction was not significant. There was also a concentration dependent effect on the antifungal activity, S4 being more effective than S1, on each of the fungi tested. Alternaria sp. was the most responsive fungal pathogen especially at 120 concentration units. These results show that the biosynthesized AgNPs have antimicrobial activity and that this effect is formulation-dependent and concentration-dependent, thus warranting further study to be used as antimicrobial materials in biomedical and agricultural applications. The analysis provides a reproducible comparative framework.
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